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Insects Unlikely Vectors for E. coli in Greens
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2010-10-08
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#E. coli O157#leafy greens#University of Georgia
Insects Unlikely Vectors for E. coli in Greens
by Dan Flynn | Oct 08, 2010
What happens to lettuce leaves and other leafy greens that become contaminated with E. coli O157:H7 and then suffer insect or mechanical damage?
Can the bacteria then become internalized, in other words, get inside the greens and become impossible to wash off?
That's what a dozen University of Georgia researchers tried to find out. The results of their study have been published in the current issue of the Journal of Food Protection.
"Environmental pests may serve as reservoirs and vectors of zoonotic pathogens to leafy greens," the study abstract explained, "however, it is unknown whether insect pests feeding on plant tissues could redistribute these pathogens on the surface of leaves to internal sites."
After using various means to apply E. coli to the tops and undersides of lettuce and spinach leaves, through mist, droplets and by inoculation, the researchers from UG's Department of Crop and Soil Sciences then exposed the greens to cabbage loopers, aphids, whiteflies, and thrips. They later found that:
-No internalized E. coli O157:H7 was detected in individual lettuce leaves 48 hours after being inoculated with a lower amount of pathogens per leaf.
-In contrast, pathogen internalization was observed 48 hours after leaves were spray inoculated with a much higher number of E. coli O157:H7 bacteria.
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