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OSKM아닌 새로운 조합 , Sall4, Nanog, Esrrb, and Lin28 (SNEL)

분류 해외신기술 작성자 운영자
등록일 2014-09-17 조회수 2826
출처 http://phys.org/news/2014-09-scientists-therapy-grade-stem-cells-cocktail.html
관련 URL http://www.ncbi.nlm.nih.gov/pubmed/25192464
첨부파일

 

Scientists create therapy-grade stem cells using new cocktail to reprogram adult cells

 

Cell Stem Cell. 2014 Sep 4;15(3):295-309. doi: 10.1016/j.stem.2014.07.003.

The Developmental Potential of iPSCs Is Greatly Influenced by Reprogramming Factor Selection.
Buganim Y1, Markoulaki S2, van Wietmarschen N3, Hoke H4, Wu T5, Ganz K2, Akhtar-Zaidi B2, He Y6, Abraham BJ2, Porubsky D3, Kulenkampff E2, Faddah DA4, Shi L2, Gao Q2, Sarkar S2, Cohen M2, Goldmann J2, Nery JR6, Schultz MD6, Ecker JR6, Xiao A5, Young RA7, Lansdorp PM8, Jaenisch R9. Author information
Abstract
Induced pluripotent stem cells (iPSCs) are commonly generated by transduction of Oct4, Sox2, Klf4, and Myc (OSKM) into cells. Although iPSCs are pluripotent, they frequently exhibit high variation in terms of quality, as measured in mice by chimera contribution and tetraploid complementation. Reliably high-quality iPSCs will be needed for future therapeutic applications. Here, we show that one major determinant of iPSC quality is the combination of reprogramming factors used. Based on tetraploid complementation, we found that ectopic expression of Sall4, Nanog, Esrrb, and Lin28 (SNEL) in mouse embryonic fibroblasts (MEFs) generated high-quality iPSCs more efficiently than other combinations of factors including OSKM. Although differentially methylated regions, tran number of master regulators, establishment of specific superenhancers, and global aneuploidy were comparable between high- and low-quality lines, aberrant gene expression, trisomy of chromosome 8, and abnormal H2A.X deposition were distinguishing features that could potentially also be applicable to human.


Jaenisch group의 Cell stem cell 논문,
이전에 발표한 논문을 근거로 하여 ips를 만드는 새로운 유전자 조합을 개발
Oct4가 포함되지 않은 조합이라는 것에 의미가 있음.
하지만, 아직은 mouse 결과이며 human은 시도중이라고 함.

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