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Biocatalysis encompasses the use of enzymes or whole cell systems for effecting the conversion of readily available, inexpensive starting materials to high value products. Enzymes frequently display exquisite selectivity, particularly chemo-, enantio- and regioselectivity, making them attractive catalysts for a wide range of chemical transformations. Enzymes also typically operate under mild conditions of pH and temperature leading to the formation of products of high purity. Modern tools of protein discovery and engineering aid the development of novel biocatalysts and their tailor-designed implementation into industrial processes. Consequently, they find wide application in the production of pharmaceutical intermediates, fine chemicals, agrochemicals, novel materials, diagnostics, biofuels and performance chemicals. The Biocatalysis Gordon Conference highlights the best science, technologies and case studies responsible for the understanding, development and incorporation of biocatalysts around the world.